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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Mucolipin-1 (F8F9Q) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Mucolipin-1 (F8F9Q) Rabbit mAb #27748

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: Mucolipin-1 (F8F9Q) Rabbit mAb
Western blot analysis of extracts from various cell lines using Mucolipin-1 (F8F9Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of mucolipin-1 protein in MCF7 and Ramos cells is consistent with the predicted expression pattern.
1/3

To Purchase # 27748

Cat. # Size Qty. Price
27748T 20 µl
$156
27748S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 35-40, 65
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Mucolipin-1 (F8F9Q) Rabbit mAb recognizes endogenous levels of total mucolipin-1 protein. This antibody detects the carboxyl terminal fragment produced following posttranslational cleavage.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human mucolipin-1 protein.

Background

Mucolipin-1 (MCOLN1, TRPML1), a member of the transient receptor potential ion channel superfamily, is a lysosomal/late endosome Ca2+ efflux channel that is important in lysosomal biogenesis (1,2). Mutations in the mucolipin-1 gene are responsible for the human lysosomal storage disease mucolipidosis type IV (MLIV), an autosomal recessive neurodegenerative disease (3). Mucolipin-1 is synthesized as a 580-amino acid protein with six transmembrane domains but can undergo cleavage in the long luminal loop between the first two transmembrane domains (4,5). Mucolipin-1 activity has been linked to autophagy and lysosomal biogenesis. Calcium influx through mucolipin-1 activates CaMKKβ and AMPK, which activate the autophagy kinase ULK1 (6). Calcium influx is also associated with activation of the calcium-dependent phosphatase calcineurin which can trigger activation of TFEB, a key transcription factor regulating autophagy and lysosomal biogenesis (7,8). Mucolipin-1 activity is upregulated by cellular stress, including reactive oxygen species (ROS) (7). Phosphorylation of mucolipin-1 by mTOR results in reduced channel activity and autophagic flux (9). Loss of mucolipin-1 or pharmacological inhibition suppresses autophagy and can reduce tumor growth and metastasis in non-small cell lung cancer and triple-negative breast cancer, suggesting the use of mucolipin-1 antagonists for cancer therapy (10-12).

Database Links

UniProt ID: Q9GZU1


Entrez-Gene Id: 57192


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