Render Target: STATIC
Render Timestamp: 2024-11-20T11:56:54.635Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:16:43.366
Product last modified at: 2024-09-13T07:01:46.945Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MURF1 Antibody #4305

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  • WB

Inquiry Info. # 4305

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    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 41
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MURF1 Antibody detects endogenous levels of total MURF1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MURF1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    Muscle-specific RING finger protein 1 (MURF1) is an E3 ubiquitin ligase that promotes muscle cell protein degradation during muscle atrophy (1). In response to conditions that promote muscle atrophy, elevated skeletal muscle MURF1 facilitates the ubiquitination and degradation of thick filaments components (2). Cardiac muscle MURF1 promotes degradation of cardiomyocyte troponin I and inhibits PKCε-mediated cardiac hypertrophy (3,4) MURF1 also regulates the degradation of the large sarcomeric protein titin, the transcriptional regulator GMEB1, the SUMO conjugating enzyme UBC9, and the ubiquitin peptidase USP13 (5). Binding of FoxO1 and the glucocorticoid receptor to adjacent sites within the MURF1 promoter region activates transcription of this E3 ubiquitin ligase (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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