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Inquiry Info. # 4305

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	41
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MURF1 Antibody detects endogenous levels of total MURF1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MURF1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Muscle-specific RING finger protein 1 (MURF1) is an E3 ubiquitin ligase that promotes muscle cell protein degradation during muscle atrophy (1). In response to conditions that promote muscle atrophy, elevated skeletal muscle MURF1 facilitates the ubiquitination and degradation of thick filaments components (2). Cardiac muscle MURF1 promotes degradation of cardiomyocyte troponin I and inhibits PKCε-mediated cardiac hypertrophy (3,4) MURF1 also regulates the degradation of the large sarcomeric protein titin, the transcriptional regulator GMEB1, the SUMO conjugating enzyme UBC9, and the ubiquitin peptidase USP13 (5). Binding of FoxO1 and the glucocorticoid receptor to adjacent sites within the MURF1 promoter region activates transcription of this E3 ubiquitin ligase (6).

Database Links

UniProt ID: Q969Q1

Entrez-Gene Id: 84676

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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MURF1 Antibody #4305