Render Target: STATIC
Render Timestamp: 2024-11-21T12:58:14.074Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:55.440
Product last modified at: 2024-11-03T13:45:07.284Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MYO1D (E6T6Q) Rabbit mAb #90307

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 115
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MYO1D (E6T6Q) Rabbit mAb recognizes endogenous levels of total MYO1D protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro990 of human MYO1D protein.

    Background

    MYO1D (myosin 1D, myosin 1d) is a class I myosin small motor protein with the ability to simultaneously bind to actin filaments and cellular membranes, coupling membrane and cytoskeletal movement. Like other family members, MYO1D contains an N-terminal motor domain for actin binding, followed by two calmodulin-binding domains (IQ domains), and a C-terminal tail homology (TH1) for interaction with membrane localized proteins (1). MYO1D controls the planar cell polarity (PCP) pathway by coupling actin filament and PCP proteins to regulate cellular orientation. Using this mechanism, the protein controls cellular left-right asymmetry and direction of cilia cell movement (2-4). MYO1D also regulates myelin membrane dynamics by interacting with myelin membrane proteins (CNP and PLP) to maintain normal morphology of oligodendrocytes (5-7). The C-terminal domain of MYO1D has been shown to bind the EGFR family kinase domain (8). This interaction is required for MYO1D anchoring of EGFR family RTKs in the membrane/cytoskeleton structure for ligand activation (9). Knockdown of MYO1D suppresses cancer growth, invasion, and downstream signaling (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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