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MyoD1 Antibody #12344

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Western Blotting Image 1: MyoD1 Antibody
Western blot analysis of extracts from RH30 and RD cells using MyoD1 Antibody.
This product is discontinued

Inquiry Info. # 12344

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Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MyoD1 Antibody recognizes endogenous levels of total MyoD1 protein. This antibody also detects a 142 kDa protein of unknown origin.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly228 of human MyoD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Myoblast determination protein 1 (MyoD1), also called myogenic factor 3 (Myf3), is a member of the MyoD family of muscle specific bHLH transcription factors (1). This family is responsible for controlling specification of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types (2,3). Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins (4-6). Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways (5-7).

Product #12344
Citations: 22

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Showing 22 Citations
Applications:
Unspecified
Reactivity:
Unspecified
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
1
Journal:
Proc Natl Acad Sci U S A
Author:
Eric M Desjardins, et al.
Year:
2022
Applications:
Western Blotting (WB)
Reactivity:
Mus musculus (Mouse)
Journal:
Genomics Proteomics Bioinformatics
Author:
Yuejing Jiang, et al.
Year:
2022
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
Applications:
Unspecified
Reactivity:
Unspecified
For Research Use Only. Not for Use in Diagnostic Procedures.
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