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Render Timestamp: 2024-11-14T10:45:50.746Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:53:35.945
Product last modified at: 2024-09-30T08:02:14.078Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit mAb #12975

Filter:
  • WB

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 20
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit mAb recognizes endogenous levels of total myosin light chain 2v (cardiac isoform) protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse myosin light chain 2v (cardiac isoform) protein.

    Background

    Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms with varying tissue distribution. Smooth muscle MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). These phosphorylation events are correlated with myosin ATPase activity and smooth muscle contraction (2). Striated muscle contraction is regulated by the troponin-tropomyosin complex in thin actin filaments and by binding of Ca2+ to troponin C (3). Two types of myosin light chain are expressed in the heart, with myosin light chain 2v (MYL2, MLC-2v) expression restricted to the ventricles and myosin light chain 2a (MYL7, MLC-2a) found specific to the atria. Mutations in the corresponding MYL2 gene are found in patients diagnosed with a form of hypertrophic cardiomyopathy characterized by thickening of the mid-left ventricle.

    Database Links

    UniProt ID: P51667


    Entrez-Gene Id: 17906


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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