N1-Methyladenosine (m1A) (E8S7H) Rabbit mAb #37739
- DB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- DB-Dot Blot
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| RNA Dot Blot | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
m1A, much like m6A, can be erased by various enzymes. ALKBH1 has been shown to erase the m1A mark on tRNAs, resulting in reduced translational initiation, which responds to the availability of glucose (11). ALKBH3 has also been shown to erase m1A marks from mRNA transcripts (8).
- Meyer, K.D. and Jaffrey, S.R. (2017) Annu Rev Cell Dev Biol 33, 319-342.
- Desrosiers, R. et al. (1974) Proc Natl Acad Sci U S A 71, 3971-5.
- Basavappa, R. and Sigler, P.B. (1991) EMBO J 10, 3105-11.
- Anderson, J. et al. (2000) Proc Natl Acad Sci U S A 97, 5173-8.
- Ozanick, S. et al. (2005) RNA 11, 1281-90.
- Peifer, C. et al. (2013) Nucleic Acids Res 41, 1151-63.
- Waku, T. et al. (2016) J Cell Sci 129, 2382-93.
- Safra, M. et al. (2017) Nature 551, 251-255.
- Grozhik, A.V. and Jaffrey, S.R. (2017) Nature 551, 174-176.
- Liu, F. et al. (2016) Cell 167, 816-828.e16.
- Li, X. et al. (2016) Nat Chem Biol 12, 311-6.
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