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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. N6-mA Methyltransferase Antibody Sampler Kit

N6-mA Methyltransferase Antibody Sampler Kit #69159

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The N6-mA Methyltransferase Antibody Sampler Kit provides an economical means of detecting N6-mA methyltransfearse proteins using control antibodies against METTL3, METTL14, WTAP, and Virilizer. The kit contains enough primary antibodies to perform at least two western blot experiments.

    Specificity / Sensitivity

    Each antibody in the N6-mA Methyltransferase Antibody Sampler Kit detects endogenous levels of its target protein.

    Source / Purification

    METTL14, METTL3, and Virilizer monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu297 of human METTL3 protein, Pro130 of human METTL14 protein, and Pro229 of human Virilizer protein. The WTAP polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human WTAP protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Methyltransferase-like protein 3 (METTL3) and methyltransferase-like protein 14 (METTL14) are the two catalytic subunits of an N6-methyltransferase complex that methylates adenosine residues in RNA (1). Methylation of adenosine residues regulates mRNA splicing, processing, translation efficiency, editing and stability, in addition to regulating primary miRNA processing, and is critical for proper regulation of the circadian clock, embryonic stem cell self-renewal, immune tolerance, response to various stimuli, meiosis and mouse fertility (2,3). In this complex, METTL3 functions as the catalytic methyltransferase subunit and METTL14 functions as the target recognition subunit by binding to RNA (4). In addition, the Wilms' tumor 1-associating protein (WTAP) functions as a regulatory subunit and is required for accumulation of the complex to nuclear speckles, which are sites of RNA processing (5). Several studies suggest a role for this complex in cancer. METTL3 expression is elevated in lung adenocarcinoma where it promotes growth, survival, and invasion of human lung cancer cells (6). In addition, WTAP is overexpressed in a number of different cancers and positively regulates cell migration and invasion in glioblastoma and cholangiocarcinoma (7,8).

    Virilizer is an additional protein subunit of the METTL3/METTL14 N6-methyltransferase complex and is required for methyltransferase activity (9). As part of the WTAP complex, Virilizer functions to regulate sex determination in Drosophila melanogaster through splicing of Sex-lethal (Sxl) mRNA transcripts (10,11).

    Database Links

    UniProt ID: Q69YN4 , Q86U44 , Q15007 , Q9HCE5


    Entrez-Gene Id: 25962 , 56339 , 9589 , 57721


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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