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Render Timestamp: 2024-12-20T11:07:48.654Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:58.620
Product last modified at: 2024-12-17T18:48:25.927Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Na Channel β2 Subunit (D6L5Y) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Na Channel β2 Subunit (D6L5Y) Rabbit mAb #13966

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 37-41
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Na channel β2 subunit (D6L5Y) Rabbit mAb recognizes endogenous levels of total Na channel β2 subunit protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu90 of mouse Na channel β2 subunit protein.

    Background

    Mammalian voltage-gated sodium channels (VGSCs) are composed of a pore-forming α subunit and one or more regulatory β subunits (1). Four separate genes (SCN1B-SCN4B) encode the five mammalian β subunits β1, β1B, β2, β3, and β4. In general, β subunit proteins are type I transmembrane proteins, with the exception of secreted β1B protein (reviewed in 2). β subunits regulate α subunit gating and kinetics, which controls cell excitability (3,4). Sodium channel β subunits also function as Ig superfamily cell adhesion molecules that regulate cell adhesion and migration (5,6). Additional research reveals sequential processing of β subunit proteins by β-secretase (BACE1) and γ secretase, resulting in ectodomain shedding of β subunit and generation of an intracellular carboxy-terminal fragment (CTF). Generation of the CTF is thought to play a role in cell adhesion and migration (7,8). Multiple studies demonstrate a link between β subunit gene mutations and a number of disorders, including epilepsy, cardiac arrhythmia, multiple sclerosis, neuropsychiatric disorders, neuropathy, inflammatory pain, and cancer (9-13).
    The sodium channel β2 subunit (SCN2B) plays a role in the assembly and functioning of cell surface sodium channels (14). Mutations in the corresponding SCN2B gene may be associated with a form of atrial fibrillation and at least one identified case of Brugada syndrome (15,16). Research using a rat model of spared nerve injury (SNI) suggests that the β2 subunit may be involved in the generation of neuropathic pain (17).
    1. Catterall, W.A. (1992) Physiol Rev 72, S15-48.
    2. Catterall, W.A. (2012) J Physiol 590, 2577-89.
    3. Isom, L.L. et al. (1992) Science 256, 839-42.
    4. Brackenbury, W.J. and Isom, L.L. (2011) Front Pharmacol 2, 53.
    5. Isom, L.L. et al. (1995) Cell 83, 433-42.
    6. Malhotra, J.D. et al. (2000) J Biol Chem 275, 11383-8.
    7. Wong, H.K. et al. (2005) J Biol Chem 280, 23009-17.
    8. Kim, D.Y. et al. (2005) J Biol Chem 280, 23251-61.
    9. Wallace, R.H. et al. (1998) Nat Genet 19, 366-70.
    10. Lopez-Santiago, L.F. et al. (2007) J Mol Cell Cardiol 43, 636-47.
    11. Chioni, A.M. et al. (2009) Int J Biochem Cell Biol 41, 1216-27.
    12. O'Malley, H.A. et al. (2009) Mol Cell Neurosci 40, 143-55.
    13. Valdivia, C.R. et al. (2010) Cardiovasc Res 86, 392-400.
    14. Schmidt, J.W. and Catterall, W.A. (1986) Cell 46, 437-44.
    15. Watanabe, H. et al. (2009) Circ Arrhythm Electrophysiol 2, 268-75.
    16. Riuró, H. et al. (2013) Hum Mutat 34, 961-6.
    17. Pertin, M. et al. (2005) J Neurosci 25, 10970-80.

    Database Links

    UniProt ID: O60939


    Entrez-Gene Id: 6327


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