NAE1/APPBP1 (D9I4Z) Rabbit mAb #14321
- WB
Supporting Data
| REACTIVITY | |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
NAE1/APPBP1 (NEDD8-Activating Enzyme 1/Amyloid Beta Precursor Protein Binding Protein 1) exists in a heterodimeric complex with UBA3. This complex functions as an E1 NEDD8-activating enzyme, which utilizes ATP to adenylate the C-terminal glycine of NEDD8 (12-14). Research studies suggest that inhibition of the APPBP1-UBA3 complex may be of therapeutic value for the treatment of human cancers (15,16).
- Huang, D.T. et al. (2007) Nature 445, 394-8.
- Huang, D.T. et al. (2005) Mol Cell 17, 341-50.
- Liakopoulos, D. et al. (1998) EMBO J 17, 2208-14.
- Gong, L. and Yeh, E.T. (1999) J Biol Chem 274, 12036-42.
- Wada, H. et al. (2000) J Biol Chem 275, 17008-15.
- Sakata, E. et al. (2007) Nat Struct Mol Biol 14, 167-8.
- Kawakami, T. et al. (2001) EMBO J 20, 4003-12.
- Podust, V.N. et al. (2000) Proc Natl Acad Sci USA 97, 4579-84.
- Wu, K. et al. (2002) J Biol Chem 277, 516-27.
- Amir, R.E. et al. (2002) J Biol Chem 277, 23253-9.
- Herrmann, J. et al. (2007) Circ Res 100, 1276-91.
- Walden, H. et al. (2003) Mol Cell 12, 1427-37.
- Walden, H. et al. (2003) Nature 422, 330-4.
- Huang, D.T. et al. (2004) Nat Struct Mol Biol 11, 927-35.
- Soucy, T.A. et al. (2009) Nature 458, 732-6.
- Swords, R.T. et al. (2010) Blood 115, 3796-800.
Limited Uses
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