Render Target: STATIC
Render Timestamp: 2024-12-23T11:16:36.883Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-18 16:03:12.776
Product last modified at: 2024-11-19T09:00:10.398Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NAGK (F9C7G) Rabbit mAb #10923

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 37
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NAGK (F9C7G) Rabbit mAb recognizes endogenous levels of total NAGK protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu65 of human NAGK protein.

    Background

    N-acetyl-D-glucosamine kinase (NAGK) is a member of the sugar kinase/Hsp70/actin superfamily, whose members phosphorylate certain substrates in an ATP-dependent manner (1-3). NAGK mediates the phosphorylation of N-acetylglucosamine (GlcNAc) to GlcNAc-6-phosphate, which is then used for biosynthesis of UDP-GlcNAc, a critical component of O-linked-N-acetylglucosaminylation and N-linked glycosylation (1-4). NAGK is expressed mainly in immune cells and is co-expressed with genes primarily associated with immune cell functions (4). NAGK can also phosphorylate muramyl dipeptide (MDP), triggering pro-inflammatory gene expression by NOD2 (nucleotide-binding oligomerization domain-containing protein), which is critical for maintaining a healthy intestinal barrier function (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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