Render Target: STATIC
Render Timestamp: 2024-12-20T11:24:54.162Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:27:50.135
Product last modified at: 2024-05-30T07:02:04.287Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Nanog Antibody (Mouse Specific) #8600

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Inquiry Info. # 8600

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    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 42
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Nanog Antibody (Mouse Specific) recognizes endogenous levels of total mouse nanog protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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