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XML generation date: 2025-03-07 13:11:49.761
Product last modified at: 2024-05-30T07:02:04.287Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Nanog Antibody (Mouse Specific) #8600

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Filter:
  • WB
Western Blotting Image 1: Nanog Antibody (Mouse Specific)
Western blot analysis of extracts from F9 cells using Nanog Antibody (Mouse Specific).
This product is discontinued

Inquiry Info. # 8600

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Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 42
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Nanog Antibody (Mouse Specific) recognizes endogenous levels of total mouse nanog protein.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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