Render Target: STATIC
Render Timestamp: 2024-12-26T10:59:30.504Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:47:46.210
Product last modified at: 2024-12-18T13:15:28.751Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NAT10 Antibody #66548

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 120
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NAT10 Antibody recognizes endogenous levels of total NAT10 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NAT10 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    N-acetyltransferase 10 (NAT10), also known as human N-acetyltransferase-like protein (hALP), is an acetyltransferase that increases telomerase activity through the activation of the TERT promoter (1). NAT10 localizes to the midbody in late phase mitosis to help achieve cytokinesis (2). It has been found that DNA damage can actually increase NAT10-dependent acetylation of alpha-tubulin, resulting in increased stability of the protein (2,3). This increased stability of microtubules has been linked to the sequestration of the nuclear import factor transportin-1 in the premature aging disease, progeria (4,5). NAT10 can also function as an acetyltransferase for both rRNA and mRNA (6-8). The acetylation of the 18 S rRNA helps in rRNA processing and ribosome biogenesis, and this process can be disrupted by opposing activity of the deacetylase SirT1 in response to stress (6,9). mRNA acetylation by NAT10 plays a role in translation efficiency and mRNA stability (7,8). NAT10 is overexpressed in many different cancer types, making it a good potential therapeutic target (10-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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