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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
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Product last modified at: 2024-05-30T07:15:18.537Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NCAM (CD56) Antibody #3606

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Filter:
  • WB
Western Blotting Image 1: NCAM (CD56) Antibody
Western blot analysis of extracts from human cerebellum and rat brain, using NCAM (CD56) Antibody.
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Inquiry Info. # 3606

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Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 140
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NCAM (CD56) Antibody detects endogenous levels of total NCAM protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 800 of human NCAM. Antibodies are purified by protein A and peptide affinity chromatography.

Background

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms' tumor, neuroblastoma, and rhabdomyosarcoma (2). CD56 is commonly used along with CD3 and CD16 to identify human natural killer (NK) cells (mouse NK cells do not express CD56) (3). Human NK cells are CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic NK cells, while those with low CD16 expression are immature precursors and cytokine producers (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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