NCK1 (15B9) Rabbit mAb #2319
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly300 of human NCK1.
Background
NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).
- Lehmann, J.M. et al. (1990) Nucleic Acids Res. 18, 1048.
- Chou, M.M. et al. (1992) Mol. Cell Biol. 12, 5834-5842.
- Li, W. et al. (1992) Mol. Cell Biol. 12, 5824-5833.
- Verma, R. et al. (2006) J. Clin. Invest. 116, 1346-1359.
- Rivera, G.M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9536-9541.
- Li, H. et al. (2006) Biochem. Biophys. Res. Commun. 349, 310-316.
- Bladt, F. et al. (2003) Mol. Cell Biol. 23, 4586-4597.
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