Render Target: STATIC
Render Timestamp: 2024-12-20T11:49:34.549Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:31.316
Product last modified at: 2024-09-30T08:01:57.024Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

NCK1 (5B7) Mouse mAb #12778

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Hm Mk
    SENSITIVITY Endogenous
    MW (kDa) 47
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NCK1 (5B7) Mouse mAb recognizes endogenous levels of total NCK1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to a fragment of human NCK1 protein.

    Background

    NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.