NCKIPSD (E6Z3N) Rabbit mAb #89661

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Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	83
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NCKIPSD (E6Z3N) Rabbit mAb recognizes endogenous levels of total NCKIPSD protein. This antibody is expected to recognize isoforms 1 and 3 of NCKIPSD protein. This antibody cross-reacts with a 60 kDa band of unknown origin by western blot in some cell lines.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val716 of human NCKIPSD protein.

Background

NCKIPSD (NCK-interacting protein with SH3 domain), also known as SPIN90, is a cytoskeletal adaptor protein involved in organization of the actin cytoskeleton and actin-based cell motility (1). Downregulation of NCKIPSD expression in breast cancer stroma is correlated with decreased survival. NCKIPSD regulates microtubule acetylation in cancer-associated fibroblasts (CAFs) (2). NCKIPSD also plays a role in modulating signaling in the central nervous system (3).

Database Links

UniProt ID: Q9NZQ3

Entrez-Gene Id: 51517

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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