Render Target: STATIC
Render Timestamp: 2024-12-20T12:04:14.568Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:57:37.052
Product last modified at: 2024-12-17T21:45:08.159Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NCOA4 (E8H8Z) Rabbit mAb #66849

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NCOA4 (E8H8Z) Rabbit mAb recognizes endogenous levels of total NCOA4 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly213 of human NCOA4 protein.

    Background

    Nuclear Receptor Coactivator 4 (NCOA4), also known as ARA70 or ELE1, was initially discovered as a transcriptional coactivator of androgen receptor (1,2). NCOA4 has also been implicated in regulation of peroxisome proliferator-activated receptor gamma and alpha, as well as estrogen receptor alpha (3-5). NCOA4 plays an autophagic role as a cargo receptor by shuttling ferritin to the lysosome in order to maintain iron homeostasis, which ultimately controls heme synthesis and red blood cell development (6-9). The NCOA4 gene has been shown to undergo a fusion event with the RET proto-oncogene, forming RET/PTC3 in thyroid and other types of cancers (10-13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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