NCS1 (D12D2) XP® Rabbit mAb #8237
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R Hm Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
NCS1 (D12D2) XP® Rabbit mAb recognizes endogenous levels of total NCS1 protein.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human NCS1 protein.
Background
According to affinity and function, calcium-binding proteins are separated into two classes: calcium buffers and calcium sensors. Calmodulin is a well-studied calcium sensor with well-established roles in synaptic plasticity. Neuronal calcium-sensor 1 (NCS1) is also a member of the calcium sensor family, however, its role in synaptic plasticity remains under investigation. NCS1 contains multiple EF-hand calcium-binding motifs and an amino-terminal myristoyl group (1). NCS1 has a large number of binding partners. Most of these protein interactions are calcium-dependent (e.g. dopamine D2 receptor), although some are calcium-independent (e.g. IP3 receptor) (2). In murine dentate gyrus, NCS1 promotes synaptic plasticity and rapid acquisition of spatial memory (3).
Limited Uses
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