NCX1 (D3F3H) Rabbit mAb #79350

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	110
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NCX1 (D3F3H) Rabbit mAb recognizes endogenous levels of total NCX1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to human NCX1 protein.

Background

NCX1 (Na⁺/Ca²⁺ exchanger, isoform 1) belongs to a conserved family of sodium-calcium membrane antiporter proteins that play a fundamental role in regulating intracellular calcium levels (1). NCX1 facilitates transmembrane transport of Ca²⁺ ions in exchange for Na⁺ ions in response to electrochemical gradients (2). Due to its relatively low affinity for calcium, NCX1 most commonly functions to export Ca²⁺ under acute conditions of high intracellular Ca²⁺. Notably however, NCX1 is a reversible antiporter, and can thus facilate Ca²⁺ influx under specialized physiological circumstances (3). Research studies have shown that NCX1 is particularly important for regulating intracellular Ca²⁺ levels in excitatory cell types (e.g., neurons, cardiac muscle). For example, conditional knockout of NCX1 in mouse cardiac pacemaker cells identified a critical role for NCX1 in the initiation and maintenance of cardiac rhythm (4).

Database Links

UniProt ID: P32418

Entrez-Gene Id: 6546

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For Research Use Only. Not For Use In Diagnostic Procedures.

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