Render Target: STATIC
Render Timestamp: 2024-12-30T11:04:19.310Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:33.369
Product last modified at: 2024-12-08T15:15:07.909Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NDUFB8 (E7U3O) Rabbit mAb #73951

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 19
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NDUFB8 (E7U3O) Rabbit mAb recognizes endogenous levels of total NDUFB8 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly171 of human NDUFB8 protein.

    Background

    NADH:ubiquinone oxidoreductase (complex I) is a large protein complex in the mitochondrial respiratory chain with multiple subunits. Complex I catalyzes the transfer of electrons from NADH to ubiquinone. NDUFB8 is one of the accessory subunits of mitochondrial complex I and is essential for its assembly (1). Studies show that nitration of NDUFB8 leads to necrosis mediated by RIP1 and RIP3 (2). In addition, NDUFB8 variants cause deficiency of mitochondrial complex I in patients with Leigh-like encephalomyopathy (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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