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Render Timestamp: 2024-11-21T13:13:34.619Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-05-10 22:31:13.494
Product last modified at: 2024-11-18T14:15:22.525Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Nestin (10C2) Mouse mAb

Nestin (10C2) Mouse mAb #33475

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 207-217
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:400 - 1:1600
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:6400

    Storage

    Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C.

    Protocol

    Specificity / Sensitivity

    Nestin (10C2) Mouse mAb recognizes endogenous levels of total nestin protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human nestin protein.

    Background

    Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly downregulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, and astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

    Database Links

    UniProt ID: P48681


    Entrez-Gene Id: 10763


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