Render Target: STATIC
Render Timestamp: 2024-11-21T14:06:46.519Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-05-21 22:08:19.338
Product last modified at: 2024-11-12T18:00:08.788Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Nestin (Rat-401) Mouse mAb #4760

Filter:
  • IHC
  • IF

    Supporting Data

    REACTIVITY R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Frozen) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #16983.

    Protocol

    Specificity / Sensitivity

    Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein.

    Species Reactivity:

    Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

    Background

    Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly downregulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, and astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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