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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. NETosis Antibody Sampler Kit

NETosis Antibody Sampler Kit #90144

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The NETosis Antibody Sampler Kit provides an economical means of detecting proteins involved in NETosis. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the NETosis Antibody Sampler Kit detects endogenous levels of its target protein. Myeloperoxidase (E1E7I) XP® Rabbit mAb recognizes the full-length and heavy chain subunits of human myeloperoxidase protein. Citrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb may also recognize histone H3 citrullinated at residue Arg26 but does not cross-react with any other known citrullinated or methylated arginine residues on histone H3. This antibody may react with a band of unknown identity at 38 kDa. Histone H3 (D1H2) XP® Rabbit mAb detects isoforms H3.1, H3.2, and H3.3. This antibody also detects the histone H3 variant CENP-A. This antibody does not cross-react with other core histones.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro734 of human myeloperoxidase protein, Pro130 of human Cathepsin G protein, residues near the carboxy terminus of human neutrophil elastase protein and histone H3, and residues near the amino terminus of histone H3 in which Arg17 is citrullinated.

    Background

    NETosis is a unique form of regulated cell death that is characterized by membrane rupture and the extrusion of chromatin, histones, and granular and cytoplasmic components into a web-like structure called neutrophil extracellular traps (NETs) (reviewed in 1). NETosis has been associated with host defense to pathogens as well as a number of disease states, including autoimmune diseases, thrombosis, cardiovascular diseases, and tumor progression. NETosis was identified as a response to bacterial infection and can be activated by lipopolysaccharide (LPS) as well as inflammatory pathway activators like phorbol-12-myristate-13-acetate (PMA) (2). It can occur via multiple pathways, but several key players have emerged. The calcium-dependent enzyme protein-arginine deiminase 4 (PAD4) catalyzes hypercitrullination of histones that contributes to chromatin decondensation (3,4). In addition, activation of proteases, including neutrophil elastase (ELANE), myeloperoxidase (MPO), and Cathepsin G, leads to impairment of cytoskeletal structures and degradation of histones during NETosis (5,6).



    Database Links

    UniProt ID: P68431 , P05164 , P08311 , P08246


    Entrez-Gene Id: 8350 , 4353 , 1511 , 1991


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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