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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Neuronal Marker IF Antibody Sampler Kit II

Neuronal Marker IF Antibody Sampler Kit II #24544

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Neuronal Marker IF Antibody Sampler Kit II provides an economical means for labeling cell types and cell structures by immunofluorescence (IF-F).

    Specificity / Sensitivity

    Each antibody in the Neuronal Marker IF Antibody Sampler Kit II has been validated for IF-F, recognizes only its specific target, and does not cross-react with other family members. Doublecortin (A8L1U) Rabbit mAb recognizes endogenous levels of total doublecortin protein. NeuN (D4G4O) XP® Rabbit mAb recognizes endogenous levels of total NeuN protein. GFAP (GA5) Mouse mAb detects endogenous levels of total GFAP protein. Myelin Basic Protein (D8X4Q) XP® Rabbit mAb recognizes endogenous levels of total myelin basic protein. Ki-67 (D3B5) Rabbit mAb recognizes endogenous levels of total Ki-67 protein.

    Source / Purification

    Rabbit and Mouse monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human doublecortin protein, or with recombinant protein specific to the amino terminus of human NeuN protein, or with native GFAP purified from pig spinal cord, or with a synthetic peptide corresponding to residues surrounding Ala185 of human myelin basic protein, or with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein.

    Background

    The antibodies in this kit serve as markers to determine cell types in the brain. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. Doublecortin is expressed in neuronal precursor cells and expression is downregulated as neurons mature (1). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (2). As Doublecortin is downregulated, NeuN is upregulated (1,2). GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP is commonly used by investigators as a marker for intracranial and intraspinal tumors arising from astrocytes (3). Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes (4). Ki-67 is universally expressed among proliferating cells and absent in quiescent cells. Specifically, it is detected in proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase (5).

    Database Links

    UniProt ID: P02686 , O43602 , A6NFN3 , P14136 , P46013


    Entrez-Gene Id: 4155 , 1641 , 146713 , 2670 , 4288


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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