Neuropeptide Y (D7Y5A) XP® Rabbit mAb #11976
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 4, 11 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #77100.
Protocol
Specificity / Sensitivity
Neuropeptide Y (D7Y5A) XP® Rabbit mAb recognizes endogenous levels of precursor (11 kDa) and mature (4 kDa) neuropeptide Y. This antibody does not cross-react with peptide YY or peptide YY (3-36) peptides. This antibody may cross-react with a protein of unknown origin at 80 kDa in some cell lines.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human mature neuropeptide Y.
Background
Neuropeptide Y (NPY) is a 36 amino acid peptide that belongs to the pancreatic polypeptide (PP) family, which also includes peptide YY (PYY) (1). The mature 36-residue NPY is produced from a larger pre-pro 97-residue NPY precursor through a series of cleavage reactions at dibasic sites and C-terminal amidation of the peptide product (2). NPY is widely expressed in the central nervous system (3) and exerts its action through stimulation of 5 different receptors (Y1-Y5) that belong to the G protein-coupled receptor family (4). NPY in the hypothalamus exhibits orexigenic activity through activation of Y1 and Y5 receptors (5). NPY is involved in the control of bone homeostasis, through the regulation of osteoblast activity by Y1 and Y2 receptors (6), and the regulation of testosterone secretion by activating Y1 receptor in testicular vessels (7). Research studies suggest that modulation of NPY activity and signaling represents a potential strategy for the development of appetite control and antiobesity agents (8).
- Tatemoto, K. et al. (1982) Nature 296, 659-60.
- O'Hare, M.M. and Schwartz, T.W. (1989) Cancer Res 49, 7010-4.
- Adrian, T.E. et al. (1983) Nature 306, 584-6.
- Larhammar, D. and Salaneck, E. (2004) Neuropeptides 38, 141-51.
- Lin, S. et al. (2004) Neuropeptides 38, 189-200.
- Lee, N.J. and Herzog, H. (2009) Neuropeptides 43, 457-63.
- Allen, C.D. et al. (2011) Neuropeptides 45, 55-61.
- Simpson, K.A. et al. (2009) Arq Bras Endocrinol Metabol 53, 120-8.
Limited Uses
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