NF-κB Non-Canonical Pathway Antibody Sampler Kit #4888
Product Information
Kit Usage Information
Protocols
- 2697: Western Blotting, Immunohistochemistry (Paraffin), Flow
- 4712: Western Blotting
- 4729: Western Blotting, Immunoprecipitation (Agarose)
- 4810: Western Blotting, Immunoprecipitation (Magnetic)
- 4882: Western Blotting, Immunoprecipitation (Agarose)
- 4922: Western Blotting, Immunoprecipitation (Agarose), CUT&RUN Assay
- 4994: Western Blotting
- 7074: Western Blotting
- 7076: Western Blotting
- 11930: Western Blotting, Immunofluorescence
Product Description
Background
The noncanonical pathway, triggered by BAFF, CD40L, and certain other stimuli, is based on the inducible phosphorylation and proteasome-mediated partial degradation of NF-κB2 p100 to p52, a process regulated by the NF-κB Inducing Kinase (NIK) and IKKα, but not IKKβ or IKKγ (4-6). NIK phosphorylates IKKα at Ser176/180 (6) and p100 at Ser866/870, then recruits IKKα to p100 where IKKα phosphorylates additional residues in the N- and C-terminus (8), leading to the ubiquitination and processing of p100 (9). The TNF Receptor Associated Factor molecules TRAF2 and TRAF3 have been shown to be negative regulators of the noncanonical pathway (10, 11), and their differential binding to receptors may also play a role in determining whether transduced signals activate the canonical pathway, noncanonical pathway, or both (12). TRAF3 promotes the rapid turnover of NIK in resting cells, and its activation-induced degradation is a key regulatory point in the pathway (13). This pathway is required for B cell maturation and activation, proper architecture of peripheral lymphoid tissue, and safeguards against autoimmunity (14).
- Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
- Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
- Ghosh, S. and Karin, M. (2002) Cell 109 Suppl, S81-96.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Xiao, G. et al. (2001) EMBO J 20, 6805-15.
- Ling, L. et al. (1998) Proc Natl Acad Sci USA 95, 3792-7.
- Xiao, G. et al. (2004) J Biol Chem 279, 30099-105.
- Liang, C. et al. (2006) Cell Signal 18, 1309-17.
- Xia, Z.P. and Chen, Z.J. (2005) Sci STKE 2005, pe7.
- Liao, G. et al. (2004) J Biol Chem 279, 26243-50.
- Morrison, M.D. et al. (2005) J Biol Chem 280, 10018-24.
- Qing, G. et al. (2005) J Biol Chem 280, 40578-82.
- Xiao, G. et al. (2006) Cytokine Growth Factor Rev 17, 281-93.
Limited Uses
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