NF-κB1 p105/p50 (D4P4D) Rabbit mAb #13586
- WB
- IP
- IF
- F
- ChIP
- C&R
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 Active form. 120 Precursor |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
| Chromatin IP | 1:50 |
| CUT&RUN | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #83394.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy terminus, SCF/β-TrCP-mediated processing produces the 50 kDa active form p50 (12,13).
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- Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
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- Thompson, J.E. et al. (1995) Cell 80, 573-82.
- Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
- Heissmeyer, V. et al. (2001) Mol Cell Biol 21, 1024-35.
- Orian, A. et al. (2000) EMBO J 19, 2580-91.
Limited Uses
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