Nitro-Tyrosine (D2W9T) Rabbit mAb #92212
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Nitro-Tyrosine (D2W9T) Rabbit mAb recognizes a broad range of nitrosylated tyrosine containing proteins and peptides. This antibody does not cross-react with phosphorylated tyrosine.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing nitrosylated tyrosine flanked by degenerate amino acids at positions N- and C-terminal to the modified tyrosine.
Background
Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2), and neurodegeneration (3). High levels of both superoxide and NO in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).
Limited Uses
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