Nitro-Tyrosine (D2W9T) Rabbit mAb #92212

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Supporting Data

REACTIVITY	All
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Nitro-Tyrosine (D2W9T) Rabbit mAb recognizes a broad range of nitrosylated tyrosine containing proteins and peptides. This antibody does not cross-react with phosphorylated tyrosine.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing nitrosylated tyrosine flanked by degenerate amino acids at positions N- and C-terminal to the modified tyrosine.

Background

Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2), and neurodegeneration (3). High levels of both superoxide and NO in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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