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Render Timestamp: 2025-01-22T02:08:45.545Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:58:09.664
Product last modified at: 2025-01-01T09:02:50.078Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NKRF (E9G1T) Rabbit mAb #63409

Filter:
  • WB
  • IP
  • eCLIP
Western Blotting Image 1: NKRF (E9G1T) Rabbit mAb
Western blot analysis of extracts from various cell lines using NKRF (E9G1T) Rabbit mAb.

To Purchase # 63409

Cat. #

Size

63409T
20 µl
63409S
100 µl

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 100
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • eCLIP-eCLIP 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
eCLIP 1:200
For more information about the RBP-eCLIP service please visit Eclipsebio.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NKRF (E9G1T) Rabbit mAb recognizes endogenous levels of total NKRF protein.

Species Reactivity:

Human, Mouse, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of human NKRF protein.

Background

NF-κB Repressing Factor (NKRF) is a constitutively expressed transcription factor that binds to the negative regulatory element within the promotor region of many genes that are transactivated by NF-κB (1). Indeed, research studies have found NKRF to repress transcription of IL-8/CXCL8, iNOS, CXCL10, and IFN-γ (2-4). In addition to regulating the expression of pro-inflammatory factors, NKRF has been shown to play a role in cell survival in the context of proteotoxic stress, through the regulation of rRNA processing and ribosome biogenesis (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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