Render Target: STATIC
Render Timestamp: 2024-12-26T12:01:25.000Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-18 16:03:14.790
Product last modified at: 2024-11-19T09:00:12.837Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NLRP12 (E8A3C) Rabbit mAb #39296

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100-110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NLRP12 (E8A3C) Rabbit mAb recognizes endogenous levels of total NLRP12 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln190 of human NLRP12 protein.

    Background

    NLR family pyrin domain containing 12 (NLRP12, NALP12, PYPAF7, MONARCH-1), a member of the NOD-like receptor (NLR) family, is an intracellular protein that plays a role in forming an inflammasome and can also act as a regulator of inflammatory signaling (1). NLRP12 is expressed in dendritic cells, neutrophils, granulocytes, and macrophages (2). Like other NLR family members, NLRP12 is composed of a pyrin domain (PYD), a nucleotide-binding domain (NBD), and a leucine-rich repeat (LRR) domain (2). NLRP12 functions as a negative regulator of canonical NFκB signaling by inhibiting accumulation of hyperphosphorylated IRAK1 downstream of TLR signaling (3). NLRP12 also acts as a negative regulator of non-canonical NFκB signaling by associating with NFκB-inducing kinase (NIK) and TRAF3, which leads to proteasomal degradation of NIK (4, 5). During Yersinia pestis infection, NLRP12 acts as an inflammasome component, resulting in the release of IL-1β and IL-18 (6). NLRP12 plays a role in regulating gut microbiota, reducing susceptibility to colitis (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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