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Render Timestamp: 2024-11-29T11:23:27.967Z
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XML generation date: 2024-08-01 15:24:04.268
Product last modified at: 2024-11-22T23:00:10.306Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. NME1/NDKA (D98) Antibody

NME1/NDKA (D98) Antibody #3345

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 18
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NME1/NDKA (D98) Antibody detects endogenous levels of total NME1/NDKA protein. This antibody is predicted to cross-react with NME2/NDKB protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to Asp98 of human NME1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regulates AMPKα1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16).
    1. Lacombe, M.L. et al. (2000) J Bioenerg Biomembr 32, 247-58.
    2. Tee, Y.T. et al. (2006) Taiwan J Obstet Gynecol 45, 107-13.
    3. Ishikawa, N. et al. (2003) J Bioenerg Biomembr 35, 7-18.
    4. Paravicini, G. et al. (1996) Biochem Biophys Res Commun 227, 82-7.
    5. Reymond, A. et al. (1999) Oncogene 18, 7244-52.
    6. Subramanian, C. et al. (2001) Nat Med 7, 350-5.
    7. Zhu, J. et al. (1999) Proc Natl Acad Sci USA 96, 14911-8.
    8. Otsuki, Y. et al. (2001) Proc Natl Acad Sci USA 98, 4385-90.
    9. Palacios, F. et al. (2002) Nat Cell Biol 4, 929-36.
    10. Fan, Z. et al. (2003) Cell 112, 659-72.
    11. Postel, E.H. (2003) J Bioenerg Biomembr 35, 31-40.
    12. Wagner, P.D. and Vu, N.D. (2000) Biochem J 346 Pt 3, 623-30.
    13. Kimura, N. et al. (2000) J Bioenerg Biomembr 32, 309-15.
    14. Steeg, P.S. (2004) J Natl Cancer Inst 96, E4.
    15. Crawford, R.M. et al. (2006) Mol Cell Biol 26, 5921-31.
    16. Schaertl, S. et al. (1999) J Biol Chem 274, 20159-64.

    Database Links

    UniProt ID: P15531


    Entrez-Gene Id: 4830


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