Render Target: STATIC
Render Timestamp: 2025-01-02T11:41:43.007Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-08-01 15:24:04.199
Product last modified at: 2025-01-01T09:07:17.129Z
Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. NME1/NDKA (G19) Antibody

NME1/NDKA (G19) Antibody #3338

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 18
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NM23-H1/H2 (G19) Antibody detects endogenous levels of total NM23-H1/H2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to Gly19 of human NM23-H1/H2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regulates AMPKα1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16).
    1. Lacombe, M.L. et al. (2000) J Bioenerg Biomembr 32, 247-58.
    2. Tee, Y.T. et al. (2006) Taiwan J Obstet Gynecol 45, 107-13.
    3. Ishikawa, N. et al. (2003) J Bioenerg Biomembr 35, 7-18.
    4. Paravicini, G. et al. (1996) Biochem Biophys Res Commun 227, 82-7.
    5. Reymond, A. et al. (1999) Oncogene 18, 7244-52.
    6. Subramanian, C. et al. (2001) Nat Med 7, 350-5.
    7. Zhu, J. et al. (1999) Proc Natl Acad Sci USA 96, 14911-8.
    8. Otsuki, Y. et al. (2001) Proc Natl Acad Sci USA 98, 4385-90.
    9. Palacios, F. et al. (2002) Nat Cell Biol 4, 929-36.
    10. Fan, Z. et al. (2003) Cell 112, 659-72.
    11. Postel, E.H. (2003) J Bioenerg Biomembr 35, 31-40.
    12. Wagner, P.D. and Vu, N.D. (2000) Biochem J 346 Pt 3, 623-30.
    13. Kimura, N. et al. (2000) J Bioenerg Biomembr 32, 309-15.
    14. Steeg, P.S. (2004) J Natl Cancer Inst 96, E4.
    15. Crawford, R.M. et al. (2006) Mol Cell Biol 26, 5921-31.
    16. Schaertl, S. et al. (1999) J Biol Chem 274, 20159-64.

    Database Links

    UniProt ID: P15531


    Entrez-Gene Id: 4830


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.