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XML generation date: 2024-12-06 15:01:07.258
Product last modified at: 2024-12-09T15:00:16.528Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit #76696

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit provides an economical means of detecting proteins involved in NHEJ DNA repair. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-DNA-PKcs (Ser2056) (E9J4G) Rabbit mAb recognizes endogenous levels of DNA-PKcs protein only when phosphorylated at Ser2056.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro608 of human DNA-PKcs protein, Val294 of mouse Ku70 protein, the carboxy terminus of human Ku80 protein, Leu771 of human DNA ligase IV protein, and Pro367 of human artemis protein. Phosphorylation-specific monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2056 of human DNA-PKcs protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human XLF protein. Polyclonal antibodies are purified by peptide affinity chromatography.

    Background

    DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, non-homologous end joining (NHEJ) and homologous recombination (HR). DNA repair via the HR pathway is restricted to S and G2 phases of the cell cycle, while NHEJ can occur during any phase. NHEJ machinery is also utilized in V(D)J recombination, a process that generates diversity in immunoglobulin and T cell receptor genes. Defects in both pathways have been associated with human disease, including cancer (1).
    DNA repair through the NHEJ pathway involves a core group of proteins that includes the Ku heterodimer (Ku70/Ku80), DNA-PKcs, DNA ligase IV, XRCC4, and XLF. XLF interacts with XRCC4 and promotes the ligation of DNA strands by DNA ligase IV and the ligase cofactor XRCC4. The ATP-dependent ligation of free DNA ends is the final step in the NHEJ repair pathway (2). DNA ligase IV and the endonuclease artemis suppress homologous recombination and promote NHEJ (3).

    Database Links

    UniProt ID: P12956 , Q96SD1 , P13010 , P78527 , Q9H9Q4 , P49917


    Entrez-Gene Id: 2547 , 64421 , 7520 , 5591 , 79840 , 3981


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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