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NOS (pan) Antibody

NOS (pan) Antibody #2977

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Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	130, 140, 160
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NOS (pan) Antibody detects endogenous levels of total eNOS, iNOS and nNOS protein.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ser1000 of human iNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

Database Links

UniProt ID: P29477

Entrez-Gene Id: 18126

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For Research Use Only. Not For Use In Diagnostic Procedures.

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