Notch Isoform Antibody Sampler Kit #3640
Product Information
Kit Usage Information
Protocols
- 3608: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), ChIP Magnetic
- 4147: Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
- 5276: Western Blotting, Immunoprecipitation (Magnetic)
- 5732: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 7074: Western Blotting
Product Description
Background
Constitutively activated Notch1 signaling is associated with the majority of cases of T cell acute lymphoblastic leukemia (T-ALL). The activation is either due to mutations in Notch1 itself or in the components of ubiquitin ligase complex, namely FBW7 (5-6). Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (7). Notch3 is a member of the Notch family and is processed similar to Notch1 (8). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (9-11). Recent studies indicate that Notch3 is overexpressed in many types of cancer (12-14).
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