Notch Receptor Interaction Antibody Sampler Kit #8658
Product Information
Kit Usage Information
Protocols
- 2210: Western Blotting, Immunoprecipitation (Magnetic)
- 2483: Western Blotting, Immunoprecipitation (Magnetic)
- 2588: Western Blotting
- 2589: Western Blotting, Immunoprecipitation (Magnetic)
- 2620: Western Blotting, Immunoprecipitation (Magnetic)
- 2756: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Immunofluorescence
- 4151: Western Blotting
- 5313: Western Blotting, Immunohistochemistry (Paraffin), ChIP Magnetic
- 6978: Western Blotting
- 7074: Western Blotting
Product Description
Specificity / Sensitivity
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala627 of human DLL1 protein, residues surrounding Gly93 of mouse DLL3 protein, residues surrounding Leu617 of human DLL4 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
TNF-α converting enzyme (TACE), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as “shedding". TACE is abundantly expressed in many adult tissues, but in fetal development, expression is differentially regulated (7). TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system (8).
Recombining Binding Protein, SUppressor of Hairless (RBPSUH), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (9-11).
Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (12,13). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (14-16). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (17). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (18,19).
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- Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43.
- Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
- Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
- Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
- Berdnik, D. et al. (2002) Dev Cell 3, 221-31.
- Santolini, E. et al. (2000) J Cell Biol 151, 1345-52.
- Dho, S.E. et al. (1999) J Biol Chem 274, 33097-104.
- Verdi, J.M. et al. (1999) Proc Natl Acad Sci U S A 96, 10472-6.
- Verdi, J.M. et al. (1999) Proc Natl Acad Sci U S A 96, 10472-6.
- McGill, M.A. and McGlade, C.J. (2003) J Biol Chem 278, 23196-203.
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- Reugels, A.M. et al. (2006) Dev Dyn 235, 934-48.
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