Notch4 (L5C5) Mouse mAb #2423
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 80 cleaved. 230 full-length. |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1 (5-7). The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors (6,8). The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling (9).
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- Uyttendaele, H. et al. (1996) Development 122, 2251-2259.
- Shirayoshi, Y. et al. (1997) Genes Cells 2, 213-224.
- Baron, M. (2003) Semin. Cell Dev. Biol. 14, 113-119.
- Gallahan, D. and Callahan, R. (1997) Oncogene 14, 1883-1890.
- Sun, Y. et al. (2005) Oncogene 24, 5365-5374.
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