Render Target: STATIC
Render Timestamp: 2024-12-20T12:10:58.514Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:24:17.943
Product last modified at: 2024-07-29T14:15:07.549Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NPC1L1 Antibody #5058

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NPC1L1 Antibody detects endogenous levels of total NPC1L1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human NPC1L1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    Niemann-Pick C1-like 1 (NPC1L1) is a transmembrane protein that plays a critical role in cholesterol absorption (1). It is highly expressed in small intestine and localized along the brush border in both human and mouse epithelial cells (2,3). NPC1L1 mediates cholesterol uptake via vesicular endocytosis (4). Ezetimibe, a potent cholesterol absorption inhibitor used to treat hypercholesterolemia (5), inhibits cholesterol uptake by preventing NPC1L1 from incorporating into clathrin-coated vesicles (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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