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Render Timestamp: 2024-12-20T11:27:52.408Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:26.837
Product last modified at: 2024-12-11T12:30:14.799Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NSD1 (E2U6H) Rabbit mAb #51076

Filter:
  • WB
  • ChIP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 300
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NSD1 (E2U6H) Rabbit mAb recognizes endogenous levels of total NSD1 protein. This antibody recognizes both the NSD1 long isoform (UniProt #Q96L73-1) and the short isoform (UniProt #Q96L73-2).

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NSD1 protein.

    Background

    Nuclear receptor SET domain-containing protein 1 (NSD1) is a member of the NSD family of histone methyltransferases, including WHSC1/NSD2 and WHSC1L1/NSD3. NSD1 specifically catalyzes mono- and di-methylation of histone H3 at Lys36 (H3K36me), a mark that is typically associated with transcriptionally active regions of the genome (1,2). NSD1 is expressed as two isoforms: a predominantly expressed short isoform of roughly 2,400 amino acids, and a less abundant long isoform of nearly 2,700 amino acids (3). NSD1 is required for proper embryonic development, as deletion of NSD1 is embryonic lethal in mice (4). Mutations in NSD1 are also believed to be the major cause of Sotos syndrome, an overgrowth disorder characterized by pre- and post-natal overgrowth, macrocephaly, advanced bone age, and developmental delay (5-7). NSD1 mutations have additionally been documented in two related overgrowth disorders, Weaver syndrome and Beckwith-Wiedemann syndrome, albeit less frequently (6-8). NSD1 is also mutated or has altered expression in several types of cancer. For instance, NSD1 is upregulated in metastatic prostate cancer and hepatocellular carcinoma (HCC) compared to healthy tissue (9,10). Loss-of-function mutations have also been observed for NSD1 in head and neck squamous cell carcinomas (HNSCCs), and the NSD1 gene is epigenetically silenced in neuroblastoma and glioma cells (11,12). NSD1, therefore, appears to exert tumor suppressive or promoting functions depending on cellular context, either through promoting the expression of tumor suppressor or oncogenic proteins, respectively (13). 
    For Research Use Only. Not For Use In Diagnostic Procedures.
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