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NUFIP1 Antibody

NUFIP1 Antibody #37783

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	75
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NUFIP1 Antibody recognizes endogenous levels of total NUFIP1 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NUFIP1 protein. Antibodies are purified by peptide affinity chromatography.

Background

NUFIP1 (nuclear FMRP interacting protein 1) was identified as an RNA-binding protein shuttling between the nucleus and cytoplasm, and it associates with ribosomes in functional synaptoneurosomes (1). Reports have shown that NUFIP1 can function as a receptor for starvation-induced ribophagy to maintain protein homeostasis (2). Ribophagy is a selective form of autophagy targeting ribosomes (reviewed in 3). Lysosomal levels of NUFIP1 were increased upon inhibition of autophagy. Ribophagy was dependent on the ability of ribosome-associated NUFIP1 to interact with LC3B via a conserved LC3-interacting region (LIR) domain and recruit ribosomes to the autophagosome (2). Loss of NUFIP1 or its LC3B binding ability inhibited cell survival following starvation (2).

Database Links

UniProt ID: Q9UHK0

Entrez-Gene Id: 26747

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For Research Use Only. Not For Use In Diagnostic Procedures.

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