NUFIP1 (E7E4L) Rabbit mAb #74030

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	75
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NUFIP1 (E7E4L) Rabbit mAb recognizes endogenous levels of total NUFIP1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NUFIP1 protein.

Background

NUFIP1 (nuclear FMRP interacting protein 1) was identified as an RNA-binding protein shuttling between the nucleus and cytoplasm, and it associates with ribosomes in functional synaptoneurosomes (1). Reports have shown that NUFIP1 can function as a receptor for starvation-induced ribophagy to maintain protein homeostasis (2). Ribophagy is a selective form of autophagy targeting ribosomes (reviewed in 3). Lysosomal levels of NUFIP1 were increased upon inhibition of autophagy. Ribophagy was dependent on the ability of ribosome-associated NUFIP1 to interact with LC3B via a conserved LC3-interacting region (LIR) domain and recruit ribosomes to the autophagosome (2). Loss of NUFIP1 or its LC3B binding ability inhibited cell survival following starvation (2).

Database Links

UniProt ID: Q9UHK0

Entrez-Gene Id: 26747

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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