Render Target: STATIC
Render Timestamp: 2024-12-20T12:18:13.324Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:48.220
Product last modified at: 2024-10-17T18:30:14.950Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. NUP153 (E3N3Y) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NUP153 (E3N3Y) Rabbit mAb #36936

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 180
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:200
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NUP153 (E3N3Y) Rabbit mAb recognizes endogenous levels of total NUP153 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro80 of mouse NUP153 protein.

    Background

    The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). Nucleoporin 153 kDa (NUP153) protein functions as a scaffolding element that recruits other proteins to form the “nuclear basket” on the nuclear side of the pore complex. NUP153 is essential for normal nucleocytoplasmic transport of proteins and mRNAs and is critical for the quality control and retention of unspliced mRNAs in the nucleus (1,2). NUP153 is a potential DNA-binding subunit of the NPC and is important for proper regulation of embryonic stem cell pluripotency and differentiation (1,3). Depletion of NUP153 leads to derepression of developmental genes and induction of early differentiation. NUP153 binds to Nucleoporin-Associated Regions (NARs) that are found near the transcriptional start sites of developmental genes and mediates recruitment of polycomb repressor complex 1 (PRC1) to repress transcription in embryonic stem cells (3).

    Database Links

    UniProt ID: Q80WR0


    Entrez-Gene Id: 218210


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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