Render Target: STATIC
Render Timestamp: 2024-12-20T11:53:13.168Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-05 16:01:06.504
Product last modified at: 2024-12-17T18:50:51.877Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. NUP62 (E4Y5P) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NUP62 (E4Y5P) Rabbit mAb #24588

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 62
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NUP62 (E4Y5P) Rabbit mAb recognizes endogenous levels of total NUP62 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly170 of human NUP62 protein.

    Background

    The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). Nucleoporin p62 (NUP62) is a ubiquitously-expressed 62 kDa protein that is an essential component of the NPC (3). NUP62 expression is increased in aggressive, lethal prostate cancer, and knockdown of NUP62 expression reduces cancer cell proliferation and colony formation in soft agar (4). NUP62 is also highly expressed in squamous cell carcinomas (SCCs), and depletion of NUP62 in SCC cells results in reduced proliferation and enhanced differentiation via regulation of p63 nucleo-cytoplasmic transport (5). Interestingly, NUP62 also plays a role in mitotic cell cycle progression after disassembly of the nuclear envelope and NPCs. Indeed, knockdown of NUP62 induces G2/M phase arrest, mitotic cell death, and abnormal centrosome assembly (6). A missense mutation of Q391P in NUP62 is believed to cause infantile bilateral striatal necrosis (IBSN), a rare and fatal autosomal recessive disease (7).

    Database Links

    UniProt ID: P37198


    Entrez-Gene Id: 23636


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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