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Render Timestamp: 2025-01-21T16:29:49.988Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:55:28.778
Product last modified at: 2025-01-01T09:06:17.301Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NY-ESO-1 (D1Q2U) Rabbit mAb #45437

Filter:
  • WB
  • IP
  • IF
  • F
Western Blotting Image 1: NY-ESO-1 (D1Q2U) Rabbit mAb
Western blot analysis of extracts from various cell lines using NY-ESO-1 (D1Q2U) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, NY-ESO-1 protein is not detected in either HeLa cells or Jurkat cells.

To Purchase # 45437

Cat. #

Size

45437T
20 µl
45437S
100 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 20 (monomer), 40 (dimer)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunofluorescence (Immunocytochemistry) 1:1600
Flow Cytometry (Fixed/Permeabilized) 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NY-ESO-1 (D1Q2U) Rabbit mAb recognizes endogenous levels of total NY-ESO-1 protein. This antibody cross-reacts with NY-ESO-2/LAGE-1S.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NY-ESO-1 protein, isoform 1.

Background

Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3).

New York esophageal squamous cell carcinoma-1 (NY-ESO-1) is an X-linked CTA and was first identified by serological analysis of cDNA expression libraries in esophageal carcinoma (SEREX) (4,5). Like other CTAs, NY-ESO-1 expression is repressed in normal somatic tissues but becomes derepressed in a variety of human cancer types, such as multiple myeloma, non-small cell lung carcinoma, liposarcoma, and melanoma (6,7). Although the biological function of NY-ESO-1 remains enigmatic, its tumor-restricted expression pattern and high degree of immunogenicity have positioned it as a prominent target of immunotherapy-based strategies for tumor eradication (8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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