Render Target: STATIC
Render Timestamp: 2024-12-20T11:11:04.614Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-18 16:03:15.179
Product last modified at: 2024-11-19T09:00:12.826Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

OCA-T2 (F5A7Z) Rabbit mAb #48122

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    OCA-T2 (F5A7Z) Rabbit mAb recognizes endogenous levels of total OCA-T2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human OCA-T2 protein.

    Background

    OCA-T2 (COLCA2, POU2AF3) and its paralog OCA-T1 (C11orf53) were first identified by single-cell RNA sequencing (scRNAseq) to be co-expressed with the transcription factor POU2F3, in specialized epithelial cells called tuft cells (1). Tuft cells exist as solitary chemosensory cells within mucosal epithelium and serve to coordinate interactions between immune and neuronal cells (2). Systematic gene knockdown experiments demonstrated chromatin-level interactions between OCA-T1, OCA-T2, and POU2F3 that appeared essential for tuft cell lineage determination (1). Notably, the oncogenic transformation of tuft cells has been identified as a defining feature of a distinct small cell lung cancer (SCLC) subtype known as SCLC-P, defined by their dependence on POU2F3 expression (3). In vitro studies showed that targeted knockdown of OCA-T1 and OCA-T2 suppressed growth of cultured SCLC-P cell lines, supporting a role for OCA-T1 and OCA-T2 in SCLC (4). Subsequently, it has been observed that gene fusions between EWSR1 or FUS and OCA-T2 are defining features of recently discovered aggressive sarcoma subtypes (5-7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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