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Product last modified at: 2025-03-25T12:15:17.302Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Occludin (6B8A3) Rat mAb #68534

Filter:
  • WB
  • IF
Western Blotting Image 1: Occludin (6B8A3) Rat mAb
Western blot analysis of extracts from control DLD-1 cells (lane 1) or CRISPR/Cas9 occludin knockout (KO) DLD-1 cells (lane 2) using Occludin (6B8A3) Rat mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 68534

Cat. # Size Qty. Price
68534T 20 µl
$152
68534S 100 µl
$336

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 65
Source/Isotype Rat IgG2a
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:200 - 1:800
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Occludin (6B8A3) Rat mAb recognizes endogenous levels of total occludin protein. In some human tissue lysates, this antibody detects a band of unknown origin at approximately 95 kDa by western blot.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse occludin protein.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV) (5).

Pathways

Explore pathways related to this product.


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