Occludin (E6B4R) Rabbit mAb #91131

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	65
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #24268.

Protocol

Specificity / Sensitivity

Occludin (E6B4R) Rabbit mAb recognizes endogenous levels of total occludin protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val294 of human occludin protein.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV) (5).

Database Links

UniProt ID: Q16625

Entrez-Gene Id: 100506658

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