OGDH (E1W8H) Rabbit mAb #26865

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Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	110
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

OGDH (E1W8H) Rabbit mAb recognizes endogenous levels of total OGDH protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu915 of mouse OGDH protein.

Background

2-oxoglutarate dehydrogenase (OGDH) is one of three enzymes in the α ketoglutarate dehydrogenase complex (OGDC) that is responsible for catalyzing a rate-regulating step of the tricarboxylic acid (Krebs) cycle. Together with dihydrolipoamide S-succinyltransferase (DLST) and dihydrolipoamide dehydrogenase (DLD), OGDH helps to convert 2-oxoglutarate to succinyl-CoA and CO₂ within eukaryotic mitochondria (1). Regulation of this enzyme complex is important for mitochondrial energy metabolism within cells (2). Research studies indicate that OGDH activity within the mitochondrial matrix is regulated by multiple factors, including calcium, the adenine nucleotides ATP and ADP, and NADH (2).

Database Links

UniProt ID: Q60597

Entrez-Gene Id: 18293

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For Research Use Only. Not For Use In Diagnostic Procedures.

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