Render Target: STATIC
Render Timestamp: 2024-12-16T12:32:59.872Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:16:13.059
Product last modified at: 2024-09-13T07:01:50.432Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Orai1 (D90B10) Rabbit mAb #3280

Filter:
  • WB

Inquiry Info. # 3280

Please see our recommended alternatives.

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Orai1 (D90B10) Rabbit mAb detects endogenous levels of total Orai1 protein. The antibody also cross-reacts with an unidentified protein at 78 kDa.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse Orai1.

    Background

    Calcium is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short term responses such as contraction and secretion to longer term regulation of cell growth and proliferation (1,2). Orai1 is the Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1 (3). The STIM1 and Orai1 are required for functional CRAC current and their co-expression leads to greatly amplified CRAC currents (4-7). There are three human homologues, Orai1, Orai2 and Orai3, which have been shown to be capable of forming store-operated channels (3, 5, 8). The Orai1, -2, and -3 channels are similarly inhibited by extracellular Ca2+, and Orai3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to the process of Ca2+ depotentiation (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.