Orexin Receptor 1/HCRTR1 (E5Q8B) Rabbit mAb #59242

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	43-52
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Orexin Receptor 1/HCRTR1 (E5Q8B) Rabbit mAb recognizes endogenous levels of total Orexin Receptor 1/HCRTR1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala9 of human Orexin Receptor 1/HCRTR1 protein.

Background

Orexin Receptor 1 or Hypocretin G-protein-coupled receptor-1 (OX1R and HCRTR1, respectively) is 1 of 2 orexin receptors expressed in the brain. Neurons in the hypothalamus express orexin receptors that bind to neuropeptides to modulate neuronal function (1,2). The neuropeptides that bind orexin receptors include Orexin-A (hypocretin-1) and Orexin-B (hypocretin-2). Orexin-A binds with a higher affinity to HCRTR1, whereas Orexin-B binds more selectively to HCRTR2 (2). Activation of HCRTR1 by its cognate neuropeptide drives downstream signaling events regulating several cellular processes, including increases in cytoplasmic Ca²⁺ levels (2,3). HCRTR1 has been linked to many behavioral processes, including wakefulness as well as migraines and mood disorders (4). The link of the orexin system to sleep and mood disorders make it a potential therapeutic target to combat insomnia, depression, anxiety, and addiction (3).

Database Links

UniProt ID: O43613

Entrez-Gene Id: 3061

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For Research Use Only. Not For Use In Diagnostic Procedures.

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